Method development steps may include:

Analyte structure, MW, and pKa evaluation
Solubility experiments with APIs, related substances,
         and product matrix samples
Initial column selection based on analytes in sample
Initial detection selection based on analyte
Preliminary aqueous/organic ratio optimization
Buffer and pH optimization
Ionic strength optimization
Ion pair agent selection, if appropriate
Temperature optimization
Flow rate optimization
Final aqueous/organic ratio optimization
Gradient shape optimization, if required
Column and flow rate adjustments
Detection wavelength(s) selection
DAD peak purity parameter selection, if required)
Injection volume optimization

Experience with Other Chromatographic modes:

Adsorption
Ion exchange
Mixed mode
Ion Chromatography
Steric exclusion
Gel Permeation
Hydrophobic interaction
Non-aqueous reverse phase
Partition
Chiral
Affinity
Other
HPLC Method Development
Let us assist you
email me
WPBAD, 5093 El Claro E, West Palm Beach, Fl 33415       method.development@gmail.com      wpbad.biz
Call for a quote today! (561) 601-9730
Detection Mode expertise:

Variable wavelength UV/Vis
Diode Array
Fluorescence
Electrochemical
Conductivity
Pulsed amperometry
Refractive index
Charged Aerosol Detection (CAD)
Evaporative Light Scattering
         Detector (ELSD)
Varied LC/MS detectors including
         MSD
Other

HPLC System expertise:

Agilent
Waters
Shimadzu
Dionex (HPLC/IC)
Hitachi
Perkin Elmer
Beckman
Varian
Kontron
Jasco
Other
Use of a systematic HPLC method development strategy leads to rapid, reproducible, robust, transferable, and most importantly approved methods.  We develop stability indicating methods that meets all of the criteria above but, in addition, the impurities/degradation components are detected and quantitated.  Comprehensive method development reports are written, reviewed, applicable changes made, and totally QA reviewed in preparation for submission, as needed.  The actual development will be performed at your site.
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